Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

TLS Online TPP Program

#Question id: 19536

#Section 4: Fundamentals of Biological Engineering

Which of the following type of yield is referred to as “Maximum possible yields”?

TLS Online TPP Program

#Question id: 19537

#Section 4: Fundamentals of Biological Engineering

The equation for aerobic production of acetic acid from ethanol is ____________

C2H5OH(ethanol) + O2(acetic acid) → CH3CO2H + H2O

Acetobacter aceti bacteria are added to vigorously-aerated medium containing 10 g l^-1 ethanol. After some time, the ethanol concentration is 2 g l^-1 and 7.5 g l^-1 acetic acid is produced. Calculate the Observed yield.

TLS Online TPP Program

#Question id: 19538

#Section 4: Fundamentals of Biological Engineering

The equation for aerobic production of acetic acid from ethanol is ____________

C2H5OH(ethanol) + O2(acetic acid) → CH3CO2H + H2O

Acetobacter aceti bacteria are added to vigorously-aerated medium containing 10 g l-1 ethanol. After some time, the ethanol concentration is 2 g l-1 and 7.5 g l-1  acetic acid is produced. Calculate the theoretical yield.

TLS Online TPP Program

#Question id: 19539

#Section 4: Fundamentals of Biological Engineering

How many grams of carbon is/are in one mole?

TLS Online TPP Program

#Question id: 19552

#Section 4: Fundamentals of Biological Engineering

What is the molarity of 245.0 g of H₂SO₄ dissolved in 1.000 L of solution?

TLS Online TPP Program

#Question id: 19553

#Section 4: Fundamentals of Biological Engineering

What is the molarity of 5.30 g of Na₂CO₃ dissolved in 400.0 mL solution?