Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

In blue white screening insertional activation of Beta-D-galactosidase will give rise to

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#Question id: 2076

#Section 2: General Biology

An organism with a cell wall would most likely be unable to take in materials through:

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#Question id: 5152

#General Aptitude

If the product 4864×9 P 2 is divisible by 12, the value of P is :

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#Question id: 15497

#Section 4: Fundamentals of Biological Engineering

A. aceti converts alcohol to acetic acid according to the stoichiometric relation

C₂H₅OH + O₂ 🡪CH₃COOH + H₂O

In a vigorously agitated and aerated reactor containing 20 g/l ethanol, the organism produces 16 g/l acetic acid and 2 g/l was the residual ethanol concentration. What are the theoretical and observed yields of acetic acid expressed in g/g ethanol?

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#Question id: 16789

#Section 1: Engineering Mathematics

The median of number 50, 48, 47, 48.5, 50.5, 52, 48.8, 46 will be: 

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#Question id: 12921

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:-

A. Gene probe production.              i. Novel proteins

B. Genome mapping.                       ii. PCR mutagenesis

C. Protein Engineering.                 iii. Sequence tagged sites

D. Gene Editing.                                iv. Use with blots