Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

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#Question id: 14442

#Section 1: Engineering Mathematics

if A=  the find A^-1

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#Question id: 2465

#Section 3: Genetics, Cellular and Molecular Biology

In the budding yeast S. cerevisiae, which of the following is NOT transported into the bud by myosin V?

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#Question id: 8915

#Section 6: Plant, Animal and Microbial Biotechnology

The number of legs an insect has, the number of vertebrae in a vertebral column, or the number of joints in a digit (such as a finger) are all strongly influenced by

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#Question id: 3251

#Section 3: Genetics, Cellular and Molecular Biology

Looking at 3 allele per locus and following Hardy Weinberg equilibrium what will be the expected heterozygosity if q1=0.5, q2=0.3, q3=0.2

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#Question id: 2953

#Section 3: Genetics, Cellular and Molecular Biology

If a cell in the G1 phase is fused with a cell in the S phase, then