TLS Online TPP Program

#Question id: 13052


What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. PCR would proceed normally
  2. Non-specific PCR of random templates will occur
  3. Non-specific PCR of random templates will occur
  4. The PCR reaction will not commence
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TLS Online TPP Program

#Question id: 15138

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in group 1 with an appropriate description in group 2.
Group 1                                                                Group 2
(P) SWISS PROT                                 (1) derived protein structure database 
(Q) Local alignment                          (2) protein sequence database
(R) CATH                                             (3) GAP
(S) Global alignment                        (4) Smith–Waterman algorithm

TLS Online TPP Program

#Question id: 15139

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which one of the following is NOT a structure or structurally derived database?

TLS Online TPP Program

#Question id: 15140

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Two types of pair-wise sequence alignment of the same hypothetical protein sequence fragments are illustrated in the figure below. Vertical bars between the sequences indicate the presence of identical amino acids. * symbols in Figure Y indicate residues not included in the alignment.





Which one of the following is correct?

TLS Online TPP Program

#Question id: 15141

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An alignment of two protein sequences is given below:

Sequence A         A G A A C D E V I G
Sequence B         A G E Y C D A V I G
The similarity score (%) for the above alignment will be:

TLS Online TPP Program

#Question id: 15142

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Dot matrix analysis of the amino acid sequences of lambda phage cI (horizontal sequence) and phage P22 c2 (vertical sequence) repressors is shown below. Which one of the following is correct?

TLS Online TPP Program

#Question id: 15143

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which one of the following is a depiction of the GenBank sequence entry format?