Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 15440

#Section 1: Engineering Mathematics

Which of the following is the general solution to A  & B are constants. 

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#Question id: 12996

#Section 2: General Biology

Which of the following is incorrect regarding to α-helix

a) In the α helix, the first four NH groups and last four CO groups will normally lack backbone  hydrogen bonds.

b) the length of an amino acid in α-helix reduced almost 3 times than linear structure.

c) the frequent occurrence of Pro and Gly residues favour α-helix formation.

d) dihedral (ϕ and ψ) angle should be close to -57 and -47.

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#Question id: 4916

#Section 3: Genetics, Cellular and Molecular Biology

Kappa particles indicate

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#Question id: 18085

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following methods comes under proteomics?

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#Question id: 15142

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Dot matrix analysis of the amino acid sequences of lambda phage cI (horizontal sequence) and phage P22 c2 (vertical sequence) repressors is shown below. Which one of the following is correct?