Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 27188

#Section 5: Bioprocess Engineering and Process Biotechnology

k for a zero order reaction is 2×10^-2 mol/L s. If the concentration of the reactant after 25s is 0.5 M. Calculate the initial concentration of the reactant.

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#Question id: 27189

#Section 5: Bioprocess Engineering and Process Biotechnology

How long will it take for the initial concentration of A to fall from 0.20 M to 0.075, if the rate constant for a reaction of zero-order in A is 0.035 mol L^–1 s^–1.

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#Question id: 27190

#Section 5: Bioprocess Engineering and Process Biotechnology

A substrate is consumed in a zero order reaction such that the concentration falls from 40 g/l to 20 g/l in 4 h. How long will it take the substrate to fall from 20 g/l to 2 g/l:

TLS Online TPP Program

#Question id: 27191

#Section 5: Bioprocess Engineering and Process Biotechnology

The half-life of a zero order reaction is 30 minutes. What is the concentration of the reactant left after 60 minutes?

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#Question id: 27192

#Section 5: Bioprocess Engineering and Process Biotechnology

Q) For a single substrate reaction, doubling the substrate concentration increases the rate by 10 fold. The order of the reaction is

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#Question id: 27193

#Section 5: Bioprocess Engineering and Process Biotechnology

In a first order reaction, 10% of the reactant is consumed in 25 minutes. Calculate : 

(1) The half-life period of the reaction