TLS Online TPP Program

#Question id: 13031


Use of alkaline solution in southern blotting helps in :

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. Transfer of the blot to nitrocellulose membrane
  2. Depurination of DNA fragments and hence breaking them into smaller fragments
  3. Denaturation of the DNA fragments helping in probe hybridization
  4. Binding of negatively charged DNA to the membrane
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TLS Online TPP Program

#Question id: 14202

#Section 5: Bioprocess Engineering and Process Biotechnology

In a fluidized-bed biofilm reactor, cells are attached on spherical plastic particles to form biofilms of average thickness L = 0.5 mm. The bed is used to remove carbon compounds from a waste-water stream. The feed flow rate and concentration of total fermentable carbon compounds in the feed are F = 2 l/h and S = 2000 mg/l. The diameter of the column is 10 cm. The kinetic constants of the microbial population are rm = 50 mg S/cm3. h and KS = 25 mg S/cm3 . The specific surface area of the biofilm in the reactor is 2.5 cm2 /cm3 . Assuming first-order reaction kinetics and an average effectiveness factor of n = 0.7 throughout the column, determine the required height of the column for effluent total carbon concentration of S0i = 100 mg/l.

___________________

TLS Online TPP Program

#Question id: 3290

#Section 3: Genetics, Cellular and Molecular Biology

Dog breeders maintain the purity of breeds by keeping dogs of different breeds apart when they are fertile. This kind of isolation is most similar to which of the following reproductive isolating mechanisms?

TLS Online TPP Program

#Question id: 636

#Section 2: General Biology

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

Most

Least

(A)

Valine

Aspartate

(B)

Glycine

Arginine

(C)

Arginine

Glycine

(D)

Glutamate

Arginine

TLS Online TPP Program

#Question id: 1984

#Section 2: General Biology

Which of the following is a pathway that would lead to the activation of cytotoxic T cells?

TLS Online TPP Program

#Question id: 12916

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is correct for Zoo blotting?