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TLS Online TPP Program

#Question id: 12937

Choose the incorrect statement about Yeast Artificial Chromosome vectors.

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. They are linear molecule composed of a centromere,telomeres and a replication origin termed ARS element.
  2. It is digested with restriction enzymes at the SUP4 and Bam HI sites separating the telomere sequences.
  3. It produces four arms and the foreign genomic DNA is fragmented.
  4. They are replicated in yeast cells and the external cell wall of the yeast needs to be removed to leave a spheroplast.
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TLS Online TPP Program

#Question id: 14281

#Section 5: Bioprocess Engineering and Process Biotechnology

cells are grown in a CSTR a steady state at two dilution rates 0.1 h^-1 and 0.5h^-1 and the steady state concentrations are as follows: 


if the inlet substrate concentration is 20 g/I, the cells follow monod growth kinetics and product formation kinetics is modelled as a both growth associated and non growth associated kinetics given by the equation then answer the following questions, the best estimate of maximum specific growth rate is: 
TLS Online TPP Program

#Question id: 14282

#Section 5: Bioprocess Engineering and Process Biotechnology

The oxygen transfer rate in an aerobic fermentation process does not depend on the
TLS Online TPP Program

#Question id: 14283

#Section 5: Bioprocess Engineering and Process Biotechnology

Aeration ina fermenter is expressed as VVM. What will be the VVM if air is sparged at 2000 L/min with a working volume 10 m^3? 
TLS Online TPP Program

#Question id: 14284

#Section 5: Bioprocess Engineering and Process Biotechnology

The specific productivity (qp) of an enzyme production Is fitted linearly with specific growth rate p of a fungal organism according to the equation The estimated values of constants alpha and beta are 0.0006 and 25 respectively. The enzyme production kinetics is 
TLS Online TPP Program

#Question id: 14285

#Section 5: Bioprocess Engineering and Process Biotechnology

Match the transport process with appropriate time constants given below 
Transport process                       time constants
1. Oxygen transfer                     p. V/Q
2. Heat transfer                      q. 4V(1.5ND3)
3. Flow                                     r. VpCp/UA
4. Mixing                              s. 1/KLa 
TLS Online TPP Program

#Question id: 14286

#Section 5: Bioprocess Engineering and Process Biotechnology

 When a liquid of density 1200kg/m3 and viscosity 0.01 kg m^-1s^-1 flows through a 1 cm ID pipe at 0.2m/s, the flow is referred as a