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TLS Online TPP Program
#Question id: 27355
#Section 3: Genetics, Cellular and Molecular Biology
How does the guide RNA help the cell know where to cut the DNA?
TLS Online TPP Program
#Question id: 27356
#Section 3: Genetics, Cellular and Molecular Biology
What is the role of the guide RNA?
TLS Online TPP Program
#Question id: 27359
#Section 5: Bioprocess Engineering and Process Biotechnology
A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 h and 100 mg hL-1 for the intercept and slope, respectively.
(a) The respective values of the Monod kinetic constants μm (h-1) and K. (mg L-¹) are as follows:
TLS Online TPP Program
#Question id: 27360
#Section 5: Bioprocess Engineering and Process Biotechnology
The same culture (with the m and K, values as computed above) is cultivated in a 10 L chemostat being operated with a 50 mL min-1 sterile feed containing 50 g L-1 of substrate. Assuming an overall yield coefficient of 0.3 (g- DCW) (g- substrate), the respective values of the outlet biomass and substrate concentrations are
TLS Online TPP Program
#Question id: 27361
#Section 5: Bioprocess Engineering and Process Biotechnology
A continuous stirred tank bioreactor produces 48 kg lysine.day-1. If the volumetric productivity is 2 g.l-1.h-1, the volume of the reactor is:
TLS Online TPP Program
#Question id: 27362
#Section 5: Bioprocess Engineering and Process Biotechnology
Continuous stirred tank fermenter is characterized by (biomass concentration X kg/m3, residual substrate is S kg/m3, Sf=substrate concentration in feed kg/m3, Dilution rate = D and µm>>D and Km<Sf, XD= the biomass productivity kg/m3h. If the dilution rate is doubled to 2D then the new steady state values in comparison with earlier steady state is characterized by