Damaged sieve elements are sealed off, but because of the processs that plug sieve plates pores, excuded phloem sap from wounds that sever sieve elements. Exudation of sap enhanced by the inclusion of;

#Question id: 13075

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13076

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 13077

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13078

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13079

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

#Question id: 13080

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology