Not present in all bacteria, this structure enables those that possess it to germinate after exposure to harsh conditions, such as boiling:

TLS Online TPP Program

#Question id: 27354

#Section 3: Genetics, Cellular and Molecular Biology

What does the cas9 protein do?

TLS Online TPP Program

#Question id: 27355

#Section 3: Genetics, Cellular and Molecular Biology

How does the guide RNA help the cell know where to cut the DNA?

TLS Online TPP Program

#Question id: 27356

#Section 3: Genetics, Cellular and Molecular Biology

What is the role of the guide RNA?

TLS Online TPP Program

#Question id: 27359

#Section 5: Bioprocess Engineering and Process Biotechnology

A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 h and 100 mg hL-1 for the intercept and slope, respectively.

(a) The respective values of the Monod kinetic constants μm (h-1) and K. (mg L-¹) are as follows:

TLS Online TPP Program

#Question id: 27360

#Section 5: Bioprocess Engineering and Process Biotechnology

The same culture (with the m and K, values as computed above) is cultivated in a 10 L chemostat being operated with a 50 mL min-1 sterile feed containing 50 g L-1 of substrate. Assuming an overall yield coefficient of 0.3 (g- DCW) (g- substrate), the respective values of the outlet biomass and substrate concentrations are

TLS Online TPP Program

#Question id: 27361

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous stirred tank bioreactor produces 48 kg lysine.day-1. If the volumetric productivity is 2 g.l-1.h-1, the volume of the reactor is: