A fermentation slurry containing Streptomyces kanamyceticus cells is filtered using a continuous rotary vacuum filter. 120 kg h^{- 1} slurry is fed to the filter; 1 kg slurry contains 60 g cell solids. To improve filtration rates, particles of diatomaceous-earth filter aid are added at a rate of 10 kg h^{- 1}. The concentration of kanamycin in the slurry is 0.05% by weight. Liquid filtrate is collected at a rate of 112 kg h^-1; the concentration of kanamycin in the filtrate is 0.045% (w/w). Filter cake containing cells and filter aid is continuously removed from the filter cloth.

 What percentage liquid is the filter cake?

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#Question id: 18647

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following technique used discontinuous type gel system

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#Question id: 18663

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by

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#Question id: 18668

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Enzymes and antibody can easily be detected in samples subjected to electrophoresis on cellulose acetate, by using the 

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#Question id: 18672

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Larger DNA fragments up to 2 × 10 ³ kb can be separated by using

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#Question id: 18683

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Eukaryotic mRNA can be separated by using 

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#Question id: 18685

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the items in Group 1 with an appropriate description in Group 2 

Group I	Group 2
P. UPGMA	1. Protein sequence database
Q. CLUSTALW	2. Phylogenetic Analysis
R. SWISS-PROT	3. 3-D structure visualization
S. RasMol	4. Multiple sequence alignment