#Question id: 380
#Section 4: Fundamentals of Biological Engineering
The Henderson-Hasselbalch equation:
#Question id: 14403
#Section 5: Bioprocess Engineering and Process Biotechnology
Invertase is immobilised in ion-exchange resin of average diameter 1 mm. The amount of enzyme in the beads is measured by protein assay as 0.05 kg m ^-3. 20 cm ^3 beads are packed into a small column reactor; 75 ml sucrose solution at a concentration of 16 mM is pumped rapidly through the bed. In another reactor an identical quantity of free enzyme is mixed into the same volume of sucrose solution. Assume the kinetic parameters for free and immobilised enzyme are equal: K,,, is 8.8 mM and the turnover number is 2.4 x 10 gmol glucose (g enzyme)-'s^-1. The effective diffusivity of sucrose in the ion-exchange resin is 2 x 10^-6cm2s 4. What is the rate of reaction by free enzyme?
#Question id: 164
#Section 2: General Biology
The uncommon amino acid selenocysteine has an R group with the structure —CH2—SeH (pKa = 5). In an aqueous solution, pH = 7.0, selenocysteine would:
#Question id: 15672
#Section 6: Plant, Animal and Microbial Biotechnology
#Question id: 15912
#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
Match the items in Group 1 with an appropriate description in Group 2
Group 1 Group 2
P. PSI-BLAST 1. ungapped, aligned motif
Q. CLUSTALW 2. Profiles
R. BLOCK 3. Heuristic method
S. FASTA 4. Multiple sequence alignment