TLS Online TPP Program

#Question id: 4554


In which studies have shown that phosphorylated NtrC bound at enhancers and σ54-polymerase bound at the promoter interact directly, forming a loop in the DNA between the binding sites?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. Polyacrylamide gel

  2. SDS-PAGE

  3. flourescence microscopy

  4. Electron microscopy

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TLS Online TPP Program

#Question id: 14230

#Section 5: Bioprocess Engineering and Process Biotechnology

In current service, 20 kg s^-1 cooling water at 12⁰C must be circulated through a coil inside a fermenter to maintain the temperature at 37⁰C The coil is 150 m long with pipe diameter 12 cm; the exit water temperature is 28⁰C After the inner and outer surfaces of the coil are cleaned it is found that only 13 kg s^-1 cooling water is required to control the fermentation temperature. Calculate the overall heat-transfer coefficient before cleaning. _____________

TLS Online TPP Program

#Question id: 14231

#Section 5: Bioprocess Engineering and Process Biotechnology

In current service, 20 kg s^-1 cooling water at 12⁰C must be circulated through a coil inside a fermenter to maintain the temperature at 37⁰C The coil is 150 m long with pipe diameter 12 cm; the exit water temperature is 28⁰C After the inner and outer surfaces of the coil are cleaned it is found that only 13 kg s^-1 cooling water is required to control the fermentation temperature. Calculate the overall heat-transfer coefficient before cleaning. What fraction of the total resistance to heat transfer before cleaning was due to fouling deposits?____________

TLS Online TPP Program

#Question id: 14232

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C^ - 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C^ - 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. What is the rate of heat transfer?

TLS Online TPP Program

#Question id: 14233

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C ^- 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C ^- 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. Calculate the overall heat-transfer coefficient._________

TLS Online TPP Program

#Question id: 14234

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C ^- 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C ^- 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1. Calculate the log-mean temperature difference _________

TLS Online TPP Program

#Question id: 14235

#Section 5: Bioprocess Engineering and Process Biotechnology

Nutrient medium is to be heated from 10⁰C to 28⁰C is a single-pass countercurrent shell-and-tube heat exchanger before being pumped into a fed-batch fermenter. Medium passes through the tubes of the exchanger; the shell-side fluid is water which enters with flow rate 3 x 10^4 kg h^- 1 and temperature 60⁰C pre-heated medium is required at a rate of 50 m^3 h^-1. The density, viscosity and heat capacity of the medium are the same as water; the thermal conductivity of the medium is 0.54 Wm^- 1 ⁰C^ - 1. It is proposed to use 30 steel tubes with inner diameter 5 cm; the tubes will be arranged in line. The pipe wall is 5-mm thick; the thermal conductivity of the metal is 50 Wm^- 1 ⁰C^ - 1. The maximum linear shell-side fluid velocity is estimated as 0.15 m s^-1.  Determine the heat-transfer area. ____________