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The GAP for RAS (called a RAS-GAP) works by inserting one of its arginine side chains into the Ras active site, thus stabilizing an intermediate in the
hydrolysis reaction.

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Constitutive defenses are costly to the plant. The production of secondary metabolites requires a significant energy investment derived from primary metabolism, which is then unavailable for use in growth and reproduction.

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The increase in Ca2+ concentration is transient because the Ca2+ is rapidly pumped back into the SR by an abundant, Ca2+ ATPase.

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