A brief pulse of red light will induce a response, provided that the light is sufficiently bright, and conversely, very dim light will work if the irradiation time is long enough. 

This reciprocal relationship between fluence rate and time is known as the law of reciprocity

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The mitotic CDKs phosphorylate specific nucleoporins, which causes nuclear pore complexes to dissociate during prophase. 

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A key function of mitotic CDKs, together with other protein kinases and ubiquitin-protein ligases, is to induce the formation of the mitotic spindle, also known as the mitotic apparatus.

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Centrosomes contain γ tubulin, which forms specific ringlike complexes (γ TuRCs) that, have the ability to nucleate microtubules and form a bipolar spindle

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Mitotic spindle attach to chromosomes via specialized protein structures known as kinetochores

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Kinetochores assemble on regions of chromosomal DNA marked by a centromere-specific H3 histone variant called CENP-A.