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TLS Online TPP Program
#Question id: 23173
#Unit 13. Methods in Biology
There are various drawbacks still in fluorescence microscopy such as
1. Light emitted comes from molecules above and below the plane of focus
2. Blurred image due to superposition of fluorescent images
Which of the above statement is/ are correct?
TLS Online TPP Program
#Question id: 23170
#Unit 13. Methods in Biology
Which epitope tags can be used to tag recombinant proteins?
1. FLAG
2. myc
3. TICK
Which of the following options is incorrect?
TLS Online TPP Program
#Question id: 23168
#Unit 13. Methods in Biology
What are the advantages of using secondary antibodies in indirect immunofluorescence?
1. It makes easy to choose a different conjugate no matter the primary antibody
2. Enhance detection by localizing more conjugate at the antigen
3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen
Which of the following options is correct?
TLS Online TPP Program
#Question id: 23166
#Unit 13. Methods in Biology
To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.
1. FITC
2. GFP
3. Fluorescein
4. Alexa 568
Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?
TLS Online TPP Program
#Question id: 23162
#Unit 13. Methods in Biology
Match the fluorescent dyes in column I with emission light in column II
| Column I | Column II |
| 1. Texas | a. far red |
| 2. Fluorescein | b. red |
| 3. Rhodamine | c. blue |
| 4. Cy5 | d. green |
Which of the following option comprises all correct matches?
TLS Online TPP Program
#Question id: 23155
#Unit 13. Methods in Biology
Match the following ions in column I with their relevant dyes column II.
| Column I | Column II |
| 1. K+ | a. Fura-2 |
| 2. Ca2+ | b. SNARF-1 |
| 3. H+ | c. Ion potassium green |
Which of the following options is correct?