Use the figure to answer the question below. The unlettered circle at the top of the figure shows a diploid nucleus with four chromosomes that have not yet replicated. There are two pairs of homologous chromosomes, one long and the other short. One haploid set is black, and the other is gray. The circles labeled A to E show various combinations of these chromosomes. What is the correct chromosomal condition for one daughter nucleus at telophase of mitosis? 

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#Question id: 13093

#SCPH28 | Zoology

You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                                    

You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?

In this table, DV = digested vector. DYG = digested yeast genome.

 

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#Question id: 5262

#SCPH06 I Botany

Which of the following characteristics is not true of a plasmid?

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#Question id: 3047

#SCPH06 I Botany

A rapidly growing bacterial species such as E. coli exhibits a typical phase of growth cycle in liquid nutrient broth. If a bacterial culture has starting density 1000 cells/ml in log phase and after four hours cell density is 4000 cells/ml.  What is specific growth rate per hour? 

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#Question id: 6974

#SCPH06 I Botany

Where are the stem cells that renew the epithelium of the gut found?

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#Question id: 5044

#I Life Science/ Life Sciences Group – I-V

The cell might not look different from its nearest or most distant neighbors in the embryo and show no visible signs of differentiation, but its developmental fate has become restricted, the process known as-