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#Question id: 13055
#SCPH01 Biochemistry
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following will provide least specific amplification in qPCR?
TLS Online TPP Program
#Question id: 27718
#Research Methodology
._______ are the basic building blocks of qualitative data.
TLS Online TPP Program
#Question id: 1700
#SCPH01 Biochemistry
Which of the following statements is TRUE of iNKT cells?
TLS Online TPP Program
#Question id: 678
#SCPH05 I Biotechnology
Which is not true about β-sheets?
TLS Online TPP Program
#Question id: 971
#SCPH28 | Zoology
What is the source of the nitrogen atoms 3 and 9 during the de novo biosynthesis of purines?