TLS Online TPP Program

#Question id: 19514


A cathode and an anode are the most common components of an electrochemical cell. Which of the following claims about the cathode is correct?

#SCPH05 I Biotechnology
  1. Oxidation occurs at the cathode
  2. Electrons move into the cathode
  3. Usually denoted by a negative sign
  4. Is usually made up of insulating material
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TLS Online TPP Program

#Question id: 13083

#SCPH01 Biochemistry

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

TLS Online TPP Program

#Question id: 13083

#SCPH05 I Biotechnology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

TLS Online TPP Program

#Question id: 13083

#SCPH06 I Botany

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

TLS Online TPP Program

#Question id: 13083

#SCPH28 | Zoology

The genomic DNA of an organism is digested with two restriction enzymes; one of the, PstI (CTGCA/G), while the other., MseI (T/TAA). This would generate the following 3 types of DNA fragments: 
(1) both ends cleaved by PstI (Pst-Pst), 
(2) both ends cleaved by MseI (Mse-Mse), and 
(3) one end generated by each of the two enzymes (Pst-Mse). 
the most frequent fragments in decreasing order,

TLS Online TPP Program

#Question id: 13084

#SCPH01 Biochemistry

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.

TLS Online TPP Program

#Question id: 13084

#SCPH05 I Biotechnology

Statement: In a modified version of AFLP, the selection of fragments using biotin-streptavidin binding is avoided.
Explanations: I. The fragments are ligated to the appropriate adapters and used for PCR amplification using two AFLP primers, each primer having a single selection nucleotide; this is called preamplification step.
II. The PCR products from this preamplificated step are diluted and used as template for second PCR amplification.