A researcher introduces double-stranded RNA into a culture of mammalian cells and can identify its location or that of its smaller subsections experimentally, using a fluorescent probe. When she finds that the introduced strand separates into single-stranded RNAs, what other evidence of this single-stranded RNA piece's activity would she find?

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#Question id: 18676

#SCPH01 Biochemistry

which of the following technique can be analysed hybridisation probes,  gene-cloning use as primers for DNA sequencing,  the polymerase chain reaction (PCR), use in site-directed mutagenesis and use as antisense therapeutics

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#Question id: 18677

#SCPH01 Biochemistry

Point mutations in DNA, such as occur in a range of human diseases, can be identified by 

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#Question id: 18678

#SCPH01 Biochemistry

which of the following technique can be analysed of PCR products from HIV-I allowed the identification of between 200 000 and 500 000 viral particles per cubic centimetre of serum.

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#Question id: 18679

#SCPH01 Biochemistry

Chiral compounds can be resolved using

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#Question id: 18680

#SCPH01 Biochemistry

DNA from Salmonella species, when digested with the restriction enzyme Xba I, gives around 15 fragments ranging from 25 kb to 680 kb This pattern of fragments, or ‘fingerprint’, is unique to that strain. If the same fingerprint is found from bacteria from other infected people, then it can be assumed that they were all infected from a common source. Thus, by comparing their eating habits, food sources, etc. the source of the infection can be traced to a restaurant, food item. The fingerprints are created and analysed by

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#Question id: 18681

#SCPH01 Biochemistry

Highly heterogeneous mixtures of proteins such as cell lysates or tissue extracts should be completely fractionated into individual proteins so as to obtain an overall picture of the protein composition and to enable location of individual proteins. The highest resolution is obtained