Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following true about real time PCR?

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#Question id: 344

#SCPH06 I Botany

The partial negative charge at one end of a water molecule is attracted to the partial positive charge of another water molecule. What is this attraction called?

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#Question id: 3886

#SCPH05 I Biotechnology

Which one of the following statements about the reverse transcriptases of retroviruses and the RNA replicases of other single-stranded RNA viruses, such as R17 and influenza virus, is correct?

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#Question id: 19609

#SCPH05 I Biotechnology

The correct bond order in the following species is —————.

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#Question id: 3658

#SCPH06 I Botany

In E. coli, the UvrABC endonuclease removes _____________and other bulky lesions by recognizing the distortion these produce in the double helix.

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#Question id: 4339

#SCPH01 Biochemistry

In positive control of several sugar metabolism-related operons, the cAMP receptor protein (CRP) binds to DNA to stimulate transcription. Which of the following environmental conditions causes an increase in CRP activity in stimulating transcription?