TLS Online TPP Program

#Question id: 7765


The enzyme complex or gene that maintains telomere integrity is________, which acts as an antisenescence complex. Mice and humans with its deficiencies age prematurely (Mitchell et al. 1999). Overexpressing or reactivating it in senescent cells extends longevity in mice without increasing cancer,

#SCPH01 Biochemistry
  1. telomerase
  2. p53
  3. p63
  4. RecA
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TLS Online TPP Program

#Question id: 14330

#SCPH05 I Biotechnology

A radioactive isotope decays at a rate proportional to the amount of isotope present. If the concentration of isotope is C (mg l-I), its rate of decay is:

                                                                    

A solution of the isotope 32p is used to radioactively label DNA for hybridisation studies. The half-life of 32p is 14.3 days. According to institutional safety requirements, the solution cannot be discarded until the activity is 1% its present value. How long will this take?____________________

TLS Online TPP Program

#Question id: 14331

#SCPH05 I Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 104. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water, what is the upper limit for viscosity of the suspension if adequate mixing is to be maintained?

TLS Online TPP Program

#Question id: 14332

#SCPH05 I Biotechnology

To ensure turbulent conditions and minimum mixing time during agitation with a turbine impeller, the Reynolds number must be at least 104. A stirred laboratory-scale fermenter with a turbine impeller 5 cm in diameter is operated at 800 rpm. If the density of broth being stirred is close to that of water. The mixing system is scaled up so the tank and impeller are 15 times the diameter of the laboratory equipment. The stirrer in the large vessel is operated so that the stirrer tip speed (tip speed = π N i D i) is the same as in the laboratory apparatus. How does scale-up affect the maximum viscosity. 

TLS Online TPP Program

#Question id: 14333

#SCPH05 I Biotechnology

A cylindrical bioreactor of diameter 3 m has four baffles. A Rushton turbine mounted in the reactor has a diameter one third the tank diameter and is operated at a speed of 90 rpm. The density of the fluid is approximately I g cm-3. The reactor is used to culture an anaerobic organism that does not require gas sparging. The broth can be assumed Newtonian. As the cells grow, the viscosity of the broth increases. when the viscosity is 1000 times greater than water, estimate the power required to achieve turbulence.

TLS Online TPP Program

#Question id: 14337

#SCPH05 I Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.

TLS Online TPP Program

#Question id: 14337

#SCPH05 I Biotechnology

A fermentation broth with viscosity 10 −2 Pa s and density 1000 kg m − 3 is agitated in a 2.7 m^ 3 baffled tank using a Rushton turbine with diameter 0.5 m and stirred speed 1 s ^−1. Estimate the mixing time.