Red kernel color in wheat results from the presence of at least one dominant allele of each of two independently segregating genes . Kernels on rr bb plants are white, and the genotypes R— bb and rr B— result in brown kernel color. Suppose that plants of a variety that is true breeding for red kernels are crossed with plants true breeding for white kernels produced F1 were allowed to selfing .what are F2 phenotype ratio ?

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#Question id: 5073

#SCPH28 | Zoology

The emergence of the polarity of an embryo is the result of:

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#Question id: 18614

#SCPH01 Biochemistry

The primer length affects the efficiency rate of PCR, this rate decreases as the primer length

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#Question id: 13091

#SCPH06 I Botany

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

Which colony’s plasmid do you actually want to use for your studies?

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#Question id: 10350

#SCPH05 I Biotechnology

Ammonium can be assimilated via an alternative pathway rather than primary pathway which involves the sequential actions of glutamine synthetase(GS) and glutamate synthase(GOGAT), these alternative pathway catalyzes by enzyme known as,

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#Question id: 15890

#SCPH05 I Biotechnology

 Tissue culture was developed by _______ but the concept of tissue culture was conceived by ______