Which the following statements is/are true about the stacking gel?

i. The stacking gel solution contains acrylamide.

ii. It is on the top of the separating gel.

iii. It is used to dilute the protein sample before it enters the main separating gel.

iv. It is into this gel that the wells are formed and the proteins loaded.

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#Question id: 15617

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 15617

#SCPH06 I Botany

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

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#Question id: 15617

#SCPH28 | Zoology

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1–, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac–). During an condition you have a wild type (Lac+) strain carrying a Tn5 insertion known to be near several Lac genes on the E. coli chromosome. You grow P1 phage on this strain and use the resulting phage lysate to infect the lac1– strain, selecting for kanamycin resistance (Kanr). Among 100 Kanr transductants, you find that 82 are Lac– and 18 are Lac+. Express the distance between Tn5 and the lac1– mutation as a cotransduction frequency;

TLS Online TPP Program

#Question id: 15618

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?

TLS Online TPP Program

#Question id: 15618

#SCPH06 I Botany

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?

TLS Online TPP Program

#Question id: 15618

#SCPH28 | Zoology

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?