In Benzer's intragenic mapping experiments, what event was required to allow production of infectious phage from rII mutants?

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#Question id: 13062

#SCPH01 Biochemistry

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

A restriction enzyme cleaves a circular plasmid vector to produce fragments : 1kb, 0.3kb, 2kb, 1kb and 0.3kb. How many bands will be visible on agarose gel?

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#Question id: 5050

#SCPH01 Biochemistry

A stem cell capable of producing all the cell types of a lineage(embryonic and extraembryonic) is said to be-

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#Question id: 833

#SCPH01 Biochemistry

Eubacteria Also Contain Self-Compartmentalized Proteases similar to proteosome in E.coli such as

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#Question id: 27773

#Research Methodology

________ research is concerned with qualitative phenomena.

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#Question id: 14287

#SCPH05 I Biotechnology

A chemostat is run with a feed rate of llitre/h when the volume of the reactor is also 1 litre. At steady state the doubling time of the cells in the chemostat is.....   h.