TLS Online TPP Program
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TLS Online TPP Program
#Question id: 18862
#SCPH01 Biochemistry
What is the shortest distance that the eye must go to concentrate on any object?
TLS Online TPP Program
#Question id: 2301
#SCPH01 Biochemistry
Microtubules are involved in the.
TLS Online TPP Program
#Question id: 10507
#SCPH05 I Biotechnology
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TLS Online TPP Program
#Question id: 2065
#SCPH05 I Biotechnology
Which of the following are least likely to diffuse through the phospholipid bilayer of a cell membrane?
TLS Online TPP Program
#Question id: 13054
#SCPH05 I Biotechnology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?