Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

What would the expected effect be on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?

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#Question id: 4162

#SCPH06 I Botany

Which statement is false concerning aminoacyl-tRNA synthetase?

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#Question id: 2640

#SCPH05 I Biotechnology

Which of these properties do not agree with trp operon attenuator?

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#Question id: 3615

#SCPH28 | Zoology

What are alleles?

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#Question id: 5323

#SCPH06 I Botany

An X-linked recessive gene produces red-green color blindness in humans. A woman with normal color vision whose father was color-blind marries a color-blind man. What is the probability that their son will be color-blind?

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#Question id: 15618

#SCPH01 Biochemistry

Wild type E. coli metabolizes the sugar lactose by expressing the enzyme ß-galactosidase. You have isolated a mutant that you call lac1-, which cannot synthesize ß-galactosidase and cannot grow on lactose (Lac-). During an condition  isolate  a second Lac– mutation, which you designate lac2-. Using P1 phage on this strain and use the resulting phage lysate to infect the lac2- strain, selecting for   Kanr   transductants. In this case, all 100   Kanr   transductants that are examined are Lac–. What does this result tell you about the relationship between the lac1- and lac2- mutations?