Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

#Question id: 7764

#I Life Science/ Life Sciences Group – I-V

Cellular senescence may have evolved to protect organisms against cancer. Instead of dividing out of control, the cells die. Cellular senescence appears to be regulated by several tumor suppressor genes, especially p53.what is the most appropriate reason behind this,

1) Transcription factor p53 is thought to suppress tumorigenesis by causing cell arrest and senescence in response to short telomeres, DNA damage, and viral or external signals to divide

2) This factor can stop the cell cycle, cause cellular senescence in rapidly dividing cells, instruct genes to initiate cellular apoptosis, and activate DNA repair enzymes.

3) Induction of apoptosis or cellular senescence by p53 always beneficial.

#Question id: 7764

#SCPH06 I Botany

Cellular senescence may have evolved to protect organisms against cancer. Instead of dividing out of control, the cells die. Cellular senescence appears to be regulated by several tumor suppressor genes, especially p53.what is the most appropriate reason behind this,

1) Transcription factor p53 is thought to suppress tumorigenesis by causing cell arrest and senescence in response to short telomeres, DNA damage, and viral or external signals to divide

2) This factor can stop the cell cycle, cause cellular senescence in rapidly dividing cells, instruct genes to initiate cellular apoptosis, and activate DNA repair enzymes.

3) Induction of apoptosis or cellular senescence by p53 always beneficial.

#Question id: 7764

#SCPH28 | Zoology

Cellular senescence may have evolved to protect organisms against cancer. Instead of dividing out of control, the cells die. Cellular senescence appears to be regulated by several tumor suppressor genes, especially p53.what is the most appropriate reason behind this,

1) Transcription factor p53 is thought to suppress tumorigenesis by causing cell arrest and senescence in response to short telomeres, DNA damage, and viral or external signals to divide

2) This factor can stop the cell cycle, cause cellular senescence in rapidly dividing cells, instruct genes to initiate cellular apoptosis, and activate DNA repair enzymes.

3) Induction of apoptosis or cellular senescence by p53 always beneficial.

#Question id: 7765

#SCPH01 Biochemistry

The enzyme complex or gene that maintains telomere integrity is________, which acts as an antisenescence complex. Mice and humans with its deficiencies age prematurely (Mitchell et al. 1999). Overexpressing or reactivating it in senescent cells extends longevity in mice without increasing cancer,

#Question id: 7765

#I Life Science/ Life Sciences Group – I-V

The enzyme complex or gene that maintains telomere integrity is________, which acts as an antisenescence complex. Mice and humans with its deficiencies age prematurely (Mitchell et al. 1999). Overexpressing or reactivating it in senescent cells extends longevity in mice without increasing cancer,

#Question id: 7765

#SCPH06 I Botany

The enzyme complex or gene that maintains telomere integrity is________, which acts as an antisenescence complex. Mice and humans with its deficiencies age prematurely (Mitchell et al. 1999). Overexpressing or reactivating it in senescent cells extends longevity in mice without increasing cancer,