Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 3266

#SCPH06 I Botany

One species mates at the season when daylight is increasing from 13 hours to 13 hours, 15 minutes; another species mates at the season when daylight is increasing from 14 hours to 14 hours, 15 minutes. The isolating mechanism is

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#Question id: 1081

#SCPH01 Biochemistry

Which of the following is a type of local signaling in which a cell secretes a signal molecule that affects neighboring cells?

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#Question id: 11094

#I Life Science/ Life Sciences Group – I-V

CO2 is more easily transferred into water across respiratory membranes compared to O2 because CO2

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#Question id: 4848

#SCPH12 I Genetics

Neurospora strain with genotype AB was cross to another strain with genotype ab. Following different ascus and their appropriate number given in table.

Distance map unit between Gene A and centromere?

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#Question id: 27150

#SCPH05 I Biotechnology

Find the x & y co-ordinate in the figure below at steady state. Here, the gas phase and liquid phase mole fraction representation is given below