Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

#Question id: 5439

#SCPH06 I Botany

Which of the following phenotypes is an example of polygenic inheritance?

#Question id: 10342

#SCPH05 I Biotechnology

Nitrate reductase is an important enzyme for nitrate assimilation. Given below some regulation of nitrate reductase activity through phosphorylation and dephosphorylation.

a) Light, and other environmental factors stimulate a protein phosphatase that dephosphorylates a key serine residue in the hinge 1 region of nitrate reductase and thereby activates the enzyme

b) In Dark, and Mg2+ stimulate a protein kinase that phosphorylates the same serine residues, which then interact with a 14-3-3 inhibitor protein, and thereby inactivate nitrate reductase

c) In Light, and Mg2+ stimulate a protein kinase that phosphorylates the serine residues, and thereby activate nitrate reductase

d) In Dark, and other environmental factors stimulate a protein phosphatase that dephosphorylates a key serine residue in the hinge 1 region of nitrate reductase and thereby inactivates the enzyme

Which of the following statements about regulation of nitrate reductase is correct?

#Question id: 14197

#SCPH05 I Biotechnology

#Question id: 4730

#SCPH01 Biochemistry

Cross is made between a pure breeding plant having red coloured male flowers with a pure breeding plant having white colored female flowers. All of these offspring are white colored flower. Upon selfing of these F1 red flower plant produced both red and white flower. Which of the following is conclusion of these cross?

#Question id: 4200

#SCPH28 | Zoology

 At the beginning of translocation, the growing peptide chain is attached to tRNA located at the __ on the ribosome.