The three genes (lacZ, lacY, and lacA) are transcribed as a single mRNA from the promoter, these genes are expressed at high levels only when

#Question id: 634

#SCPH05 I Biotechnology

An enzyme has a Vmax of 50 micromol product formed (minute x mg protein)-1 and a Km of 10 microM for the substrate. When a reaction mixture contains the enzyme and 5 microM substrate, which of the following percentages of the maximum velocity will be closest to the initial reaction rate?

#Question id: 635

#SCPH05 I Biotechnology

The enymatic rate constant (kcat/Km) of orotidine 5ʹ-phosphate decarboxylase is 6 x 10⁷ M^-1s^-1 and the nonenzymatic rate constant (kn) is 3 x 10^-16 s^-1.  What is the value of the enzymeʹs catalytic proficiency?

#Question id: 636

#SCPH05 I Biotechnology

DNA polymerase contains a lysine residue that is important for binding to DNA. Mutations were found that converted this lysine to either glutamate, glycine, valine, or arginine. Which mutations would be predicted to be the most and least harmful to the ability of the enzyme to bind DNA?

#Question id: 637

#SCPH05 I Biotechnology

Effect of temperature on enzymatic catalysis is analysed by the rate v of a chemical reaction varies with temperature according to the Arrhenius Equation which explains the sensitivity of enzyme reactions to temperature. A plot to determination of the activation energy Ea involves

#Question id: 638

#SCPH05 I Biotechnology

Match the following class of enzymes with them

#Question id: 639

#SCPH05 I Biotechnology

Match the coenzymes in column I enzymes to class of enzymes in column II