EGTA [ethylene glycol-bis(b-aminoethyl ether)- N,N,N′,N′-tetraacetic acid], a compound that can chelate (form a complex with) Ca2+, prevents Ca2+ uptake by cells and inhibits root gravitropism.

TLS Online TPP Program

#Id: 2914

#Unit 3. Fundamental Processes

The protein single-strand binding protein (SSB) stabilizes the single-stranded DNA as it is formed and modulates the helicase activity.

TLS Online TPP Program

#Id: 2915

#Unit 3. Fundamental Processes

The length of duplex DNA that usually is unwound to initiate replicati011 is probably <60 bp.

TLS Online TPP Program

#Id: 2916

#Unit 3. Fundamental Processes

HU has the capacity to bend DNA, and is involved in building the structure that leads to formation of the open complex.

TLS Online TPP Program

#Id: 2917

#Unit 3. Fundamental Processes

Input of energy in the form of ATP is required at several stages for the prepriming reaction, and it is required for unwinding DNA.

TLS Online TPP Program

#Id: 2918

#Unit 3. Fundamental Processes

Once the prepriming complex is loaded onto the replication forks, the next step is the recruitment of the primase, DnaG, which is then loaded onto the DnaB hexamer.

TLS Online TPP Program

#Id: 2920

#Unit 3. Fundamental Processes

This entails release of DnaC, which allows the DnaB helicase to become active. DnaC hydrolyzes ATP in order to release DnaB. This step marks the transition from initiation to elongation.