Heterozygous condition may produce imbalances in the amounts of gene products similar to those produced by extra gene copies.

TLS Online TPP Program

#Id: 708

#Unit 3. Fundamental Processes

seqA is part of a negative regulatory circuit that prevents origins from being remethylated.

TLS Online TPP Program

#Id: 709

#Unit 3. Fundamental Processes

A locus called dat has a large concentration of DnaA-binding sites. It binds about 80 more DnaA molecules than the origin.

TLS Online TPP Program

#Id: 710

#Unit 3. Fundamental Processes

Deletion of dat causes initiation to occur more frequently. This significantly increases the amount of DnaA available to the origin

TLS Online TPP Program

#Id: 711

#Unit 3. Fundamental Processes

DnaA has a weak intrinsic activity that converts the ATP to ADP, this is enhanced by a factor termed Hda.

TLS Online TPP Program

#Id: 712

#Unit 3. Fundamental Processes

Hda is recruited by DNA Polymerase in previous cycle and it acts to switch off DnaA, preventing a second round of replication.

TLS Online TPP Program

#Id: 713

#Unit 3. Fundamental Processes

Physical sequestration of the origin, delay in remethylation, competition for DnaA binding, hydrolysis of DnaA- bound ATP, and repression of dnaA transcription are the key steps to regulate initiation.