Presence of the s-subunit permitted recognition of authentic RNA polymerase binding sites

#Id: 928

#Unit 13. Methods in Biology

BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies

#Id: 929

#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis

#Id: 2456

#Unit 13. Methods in Biology

The term electrophoresis describes the migration of a charged particle in medium (buffer/liquid) under the influence of an electric field.

#Id: 2457

#Unit 13. Methods in Biology

In electrophoresis because of the varying charges and masses, of different molecules and particles of a mixture will migrate at different velocities under uniform force and will thus be separated into single fractions.

#Id: 2458

#Unit 13. Methods in Biology

When the potential gradient E is applied, the force F on a molecule bearing a charge of Q  sepreated  by distance d (measured in coulombs) is                  (in newton). 

#Id: 2459

#Unit 13. Methods in Biology

If the frictional force taken into the consideration (which depend on hydrodynamic size, shape of the molecule, pore size of the medium, & pore size of the medium) velocity, v , of a charged molecule in an electric field is therefore given by the equation

where f is the frictional coefficient.