Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.

TLS Online TPP Program

#Id: 5061

#Unit 3. Fundamental Processes

SeqA binds to hemimethylated DNA and is required for 13 min delaying rereplication.

TLS Online TPP Program

#Id: 5062

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and become unavailable to methylases.

TLS Online TPP Program

#Id: 5063

#Unit 3. Fundamental Processes

Replication in bacteria and in eukaryotes is licensed and permitted to occur only once per cell cycle. Each replicon is allowed to fire only once.

TLS Online TPP Program

#Id: 5064

#Unit 3. Fundamental Processes

13 minutes delaying, sequesters Oric regions in a form in which they are inaccessible to the Dam methylase and this is done by SeqA protein.

TLS Online TPP Program

#Id: 5065

#Unit 3. Fundamental Processes

SeqA mutants reduce the delay in remethylation at both oriC and dnaA. As a result, they initiate DNA replication too soon, thereby accumulating an excessive number of origins.

TLS Online TPP Program

#Id: 5066

#Unit 3. Fundamental Processes

SeqA is part of a negative regulatory circuit that prevents origins from being remethylated.