Splicing complexes

TLS Online TPP Program

#Id: 4469

#Unit 13. Methods in Biology

Resolving power or resolution is the ability to distinguish between two very closely positioned objects.

TLS Online TPP Program

#Id: 4470

#Unit 13. Methods in Biology

Resolution is improved by using shorter wavelengths of light (decreasing the value of λ) or by gathering more light (increasing either N or α).

TLS Online TPP Program

#Id: 4471

#Unit 13. Methods in Biology

The limit of resolution of a light microscope using visible light is about 0.2 μm (200 nm).

TLS Online TPP Program

#Id: 4472

#Unit 13. Methods in Biology

Phase contrast and Differential interference microscopy are used to image live cells and unstained tissues.

TLS Online TPP Program

#Id: 4473

#Unit 13. Methods in Biology

Phase-contrast microscopy generates image in which degree of darkness or brightness of a region of specimen depends on the refractive index of that region.

TLS Online TPP Program

#Id: 4474

#Unit 13. Methods in Biology

Unobstructed direct light passes through a region of the phase plate that both transmits only a small percentage of the light and changes its phase slightly