Once DNA has been melted, the trajectory of the individual strands within the enzyme is no longer constrained by the rigidity of the double helix, allowing DNA to make its 90° turn at the active site.

TLS Online TPP Program

#Id: 2923

#Unit 3. Fundamental Processes

Seq A binds to hemi methylated DNA and is required for delaying rereplication. 

TLS Online TPP Program

#Id: 2924

#Unit 3. Fundamental Processes

SeqA may interact with DnaA.

TLS Online TPP Program

#Id: 2925

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. 

TLS Online TPP Program

#Id: 2926

#Unit 3. Fundamental Processes

As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases. 

TLS Online TPP Program

#Id: 2930

#Unit 3. Fundamental Processes

The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.

TLS Online TPP Program

#Id: 2931

#Unit 3. Fundamental Processes

Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.