RNA polymerase starts most transcripts with an A, and that ribonucleotide binds the template nucleotide (T) with only two hydrogen bonds (as opposed to the three between C and G).

TLS Online TPP Program

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#Unit 3. Fundamental Processes

consensus sequence of TATAAT in which the leading TA and the final T are highly conserved. Upstream sequences around position –35 also have a region of sequence similarity, TTGACA. 

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Core enzyme binds indiscriminately to any DNA. Sigma factor reduces the affinity for sequence independent binding and confers specificity for promoter.

TLS Online TPP Program

#Id: 2346

#Unit 3. Fundamental Processes

Sigma factor reduces the affinity for sequence independent binding and confers specificity for promoter.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Sigma seems to stimulate both initiation and elongation.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Transcription Is Initiated by RNA Polymerase without the Need for a Primer known as de novo synthesis 

TLS Online TPP Program

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#Unit 3. Fundamental Processes

UACUAAC is branch point sequence for U2 type introns

CCUUAAC is branch point sequence for U12 type introns