In C. elegans, about 70% of genes are processed by the trans-splicing mechanism

TLS Online TPP Program

#Id: 6800

#Unit 3. Fundamental Processes

the RNA guide strand recruits RISC to a target RNA. It has been argued that the length of 22 nucleotides is just long enough to specify a single target sequence uniquely in the large genomes of complex eukaryotes using RNA–RNA base pairing.

TLS Online TPP Program

#Id: 6801

#Unit 3. Fundamental Processes

Binding of RISC complexes causes the bound mRNPs to associate with P bodies. Since P bodies are major sites of mRNA degradation where the decapping complex DPC1/DPC2, t5′→3′ exonuclease XRN1, and cytoplasmic exosomes are concentrated, mRNAs bound by several RISC complexes are degraded.

TLS Online TPP Program

#Id: 6802

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6803

#Unit 3. Fundamental Processes

The base pairing between miRNA and target RNA is initiated by interactions of so-called seed residues—typically the sequence between bases 2 and 9 of the 22-nucleotide miRNA

TLS Online TPP Program

#Id: 6804

#Unit 3. Fundamental Processes

Humans express four Argonaute proteins. AGO2 is the human Argonaute protein in miRNA-containing RISC complexes.

TLS Online TPP Program

#Id: 6805

#Unit 3. Fundamental Processes

AGO2 is the protein responsible for the cleavage of targetRNA. One domain of the protein is homologous to theRNase H enzymes that degrade the RNA of an RNA-DNA

hybrid