he ability of the enzyme to terminate in vitro, however, is strongly influenced by parameters such as the ionic strength and temperature at which the reaction is performed.

TLS Online TPP Program

#Id: 2637

#Unit 13. Methods in Biology

Primer extension Using primer extension one can locate the 59-end of a transcript by hybridizing an oligonucleotide primer to the RNA of interest, extending the primer with reverse transcriptase to the 5’-end of the transcript, and electrophoresing the reverse transcript to determine its size

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#Id: 8164

#Unit 2. Cellular Organization

Vesicles coated with GGA proteins and clathrin bind to proteins containing 

Asp-X-Leu-Leu and Asp-Phe-Gly-X-Φ sequences

TLS Online TPP Program

#Id: 3604

#Unit 3. Fundamental Processes

The translation machinery is composed of four primary components: mRNAs, tRNAs, aminoacyl-tRNA synthetases, and the ribosome.

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#Id: 5274

#Unit 7. System Physiology – Animal

When the potential reaches a threshold voltage of about −40 millivolts, the L-type calcium channels become “activated,” causing the action potential.

TLS Online TPP Program

#Id: 9645

#Unit 4. Cell Communication and Cell Signaling

Active Ras⋅GTP binds to the N-terminal autoinhibitory domain of Raf, altering its conformation and allowing the phosphate attached to this domain to be removed by cytosolic phosphatases, causing loss of 14-3-3 binding.