Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.

TLS Online TPP Program

#Id: 9646

#Unit 4. Cell Communication and Cell Signaling

Active Raf phosphorylates MEK on one or two serine residues in its activation loop, activating MEK kinase activity

TLS Online TPP Program

#Id: 9647

#Unit 4. Cell Communication and Cell Signaling

Constitutively active Raf kinase

a glutamic acid substitution for the valine at position 600

TLS Online TPP Program

#Id: 9648

#Unit 4. Cell Communication and Cell Signaling

MEK is a dual-specificity protein kinase

phosphorylates its target proteins on both tyrosine and serine/threonine

TLS Online TPP Program

#Id: 9649

#Unit 4. Cell Communication and Cell Signaling

In humans, there are three RAS, three Raf, two MEK, and two MAP proteins.

TLS Online TPP Program

#Id: 9650

#Unit 4. Cell Communication and Cell Signaling

Mammalian MAP kinases include  Jun N-terminal kinases and p38 kinases, which are activated by signaling pathways in response to various types of stresses

TLS Online TPP Program

#Id: 9651

#Unit 4. Cell Communication and Cell Signaling

Phosphorylation by MEK at Y-185 and T-183 in activation loop of MAP kinase leads to a marked conformational change & promotes MAP kinase activation and dimerization.