Nurturing Life Sciences
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Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.
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#Unit 2. Cellular Organization
Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA at linker DNA.
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The high-mobility group (HMG) are nonhistones and may contain Transcriptional Factors.
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Core DNA has an invariant length of 146 bp, the minimum length of DNA needed to form a stable monomeric nucleosome, and is relatively resistant to digestion by nucleases- DNA FINGER PRINTING
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The predicted mass of the nucleosome is 262 kD.
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The nucleosome consists of approximately equal masses of DNA and histones (including H1).