The PCR involves two oligonucleotide primers, 17–30 nucleotides in length, which flank the DNA sequence that is to be amplified.

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#Unit 5. Developmental Biology

Fate map, 

A diagram that “maps” the larval or adult structures onto the region of the embryo from which they arose.

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Commitment

The commitment of cells to specific cell fates & their capacity to differentiate into particular kinds of cells (cell has began in a direction towards a specific fate).

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Commitment

At this stage the cell or tissue does not appear phenotypically different from its uncommitted state, its developmental fate has become restricted.

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Stages of commitment- 

1- Specification 

2- Determination

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Cell Specification

cells become specified to a specific fate

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Specification (capable of being altered)

It is a labile phase, so it is not fixed.