The PCR involves two oligonucleotide primers, 17–30 nucleotides in length, which flank the DNA sequence that is to be amplified.

TLS Online TPP Program

#Id: 3495

#Unit 12. Applied Biology

Chromosome doubling of haploid plants produced by anther culture generates homozygous lines.

TLS Online TPP Program

#Id: 3497

#Unit 12. Applied Biology

Haploid plants can be produced in large numbers by anther and ovary cultures and from interspecific crosses.

TLS Online TPP Program

#Id: 3498

#Unit 12. Applied Biology

In protoplast fusion, protoplasts of desired strains/species are mixed in almost equal proportion by PEG induced, electrofusion and high pH-high Ca²⁺ treatments.

TLS Online TPP Program

#Id: 3499

#Unit 12. Applied Biology

Release of phenolic compounds from the cut surfaces of explants causes browning of tissue culture medium.

TLS Online TPP Program

#Id: 4230

#Unit 12. Applied Biology

Primary cultures are cells derived directly from tissues following enzymatic dissociation or from tissue fragments referred to as explants.

TLS Online TPP Program

#Id: 4231

#Unit 12. Applied Biology

Continuous cell lines are the cell lines that has gained the ability for infinite growth after transformation, which can occur spontaneously or can be chemically or virally induced.