The ‘Wallace rule’ (Lay Thein & Wallace 1986) is used to determine the appropriate stringency wash temperature: 

Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs

TLS Online TPP Program

#Id: 97

#Unit 5. Developmental Biology

Conditional specification is the process by which cells achieve their respective fates by interacting with other cells.

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#Id: 3000

#Unit 13. Methods in Biology

Crude differential centrifugation techniques can be conveniently employed to isolate
intact mitochondria and microsomes.

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#Id: 3722

#Unit 10. Ecological Principles

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#Id: 2320

#Unit 3. Fundamental Processes

Minor U12-type with GU-AG introns (hybrid type)

TLS Online TPP Program

#Id: 6510

#Unit 7. System Physiology – Animal

Tyrosine is transported into catecholamine-secreting neurons via a Na+ - dependent carrier