The ‘Wallace rule’ (Lay Thein & Wallace 1986) is used to determine the appropriate stringency wash temperature: 

Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs

TLS Online TPP Program

#Id: 1630

#Unit 4. Cell Communication and Cell Signaling

C5b component is extremely labile, is not covalently bound to the membrane like C3b and C4b, and is rapidly inactivated unless it is stabilized by the binding of C6.

TLS Online TPP Program

#Id: 1631

#Unit 4. Cell Communication and Cell Signaling

C5b67 complexes can insert into the membrane of nearby cells and mediate “innocent bystander” lysis.

TLS Online TPP Program

#Id: 1632

#Unit 4. Cell Communication and Cell Signaling

C5b, C6 and C7 complex undergoes a conformational change that exposes hydrophobic regions on the surface of the C7 component capable of inserting into the interior of the microbial membrane.

TLS Online TPP Program

#Id: 1633

#Unit 4. Cell Communication and Cell Signaling

C8 having two peptide chains: C8β and C8αϒ. Binding of C8β with C5b67 complex causes conformational change in the C8 dimer such that hydrophobic domain of C8αϒ can insert into the interior of the phospholipid membrane.

TLS Online TPP Program

#Id: 1634

#Unit 4. Cell Communication and Cell Signaling

C5b678 complex can create a small pore, 10 Å in diameter.

TLS Online TPP Program

#Id: 1635

#Unit 4. Cell Communication and Cell Signaling

MAC is the binding and polymerization of C9 to the C5b678 complex, completed MAC, which has a tubular form and functional pore diameter of 70 Å to 100 Å.