At the second cycle of PCR, primers will anneal to the 'long product' at sites where sequences complementary· to them are located, which will be much before the actual 3'-end of the long product.

TLS Online TPP Program

#Id: 719

#Unit 3. Fundamental Processes

A genome in a bacterial cell has a single replication origin and thus constitutes a single replicon.

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#Id: 720

#Unit 3. Fundamental Processes

Each haploid bacterium typically has a single chromosome, so this type of replication control is called single copy.

TLS Online TPP Program

#Id: 721

#Unit 3. Fundamental Processes

The single circular chromosomes of Sulfolobus species have three origins and thus are composed of three replicons.

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#Id: 722

#Unit 3. Fundamental Processes

Each eukaryotic chromosome contains a large number of replicons spaced unevenly throughout the chromosomes.

TLS Online TPP Program

#Id: 723

#Unit 3. Fundamental Processes

The bacterial DnaA protein is the replication initiator; it binds sequence specifically to multiple sites (dnaA boxes) in oriC.

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#Id: 724

#Unit 3. Fundamental Processes

Activity of the replication origin is controlled is DNA methylation