The two primers used in a PCR reaction have identical Tm for successful and specific amplification. 

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Ser2 phosphatase Fcp1 regenerates RNA Pol II pool

TLS Online TPP Program

#Id: 6663

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6664

#Unit 3. Fundamental Processes

Splicing is an SN2 reaction that proceeds through a pentavalent phosphorous intermediate.

TLS Online TPP Program

#Id: 6665

#Unit 3. Fundamental Processes

There are three type of Splicing Machinery

TLS Online TPP Program

#Id: 6666

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6667

#Unit 3. Fundamental Processes

The group I and group II introns have the remarkable ability to excise themselves from an RNA. This is called autosplicing, or self splicing. 

Group I introns are more common than group II introns.