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In case of nested PCR, the inner primers have a lower annealing temperature than that of the outer pair. For the initial several cycles of PCR the higher denaturation and annealing temperatures are used; as a result, only the outer pair of primer is able to prime amplification. 




#Unit 13. Methods in Biology #PCR #Part B Pointers
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#Unit 7. System Physiology – Animal

Testosterone, secreted by the Leydig cells located in the interstitium of the testis, is essential for growth and division of the testicular germinal cells.




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#Unit 8. Inheritance Biology


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#Unit 4. Cell Communication and Cell Signaling

Dominant gain-of-function mutations in proto-oncogenes and recessive loss-of-function mutations in tumor-suppressor genes are oncogenic.

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#Unit 5. Developmental Biology

SDF-2 activates the histidine phosphatase activity of DhkA, which induces spore encapsulation.

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#Unit 7. System Physiology – Animal

Agents that decrease contractility have a Negative inotropic effect.

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