In case of hotstart PCR, After the reaction mixture is prepared, primers can pair to the single-stranded regions in a nonspecific manner. 

A more attractive alternative is to cover the reaction mixture with a plug of inert wax and place the critical reagent on top of this plug. 

TLS Online TPP Program

#Id: 6626

#Unit 8. Inheritance Biology

TLS Online TPP Program

#Id: 5852

#Unit 10. Ecological Principles

TLS Online TPP Program

#Id: 10009

#Unit 4. Cell Communication and Cell Signaling

Secretion requires the addition of cholesterol and palmitic acid to the Hedgehog protein

TLS Online TPP Program

#Id: 2049

#Unit 8. Inheritance Biology

X-Linked Recessive Trait

a. Usually more males than females are affected.

b. Affected sons are usually born to unaffected mothers; thus, the trait skips generations.

c. Approximately half of a carrier (heterozygous) mother’s sons are affected.

d. Never passed from father to son.

e. All daughters of affected fathers are carriers.

TLS Online TPP Program

#Id: 9071

#Unit 4. Cell Communication and Cell Signaling

Feedback mechanisms suppress signaling by the PKA pathway:

1. Intrinsic GTPase activity of Gαs

2. PDE acts to hydrolyze cAMP to 5′-AMP

3. By BARK