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TLS Online TPP Program

#Id: 2600

Touch down PCR are derived to enhance primer binding in the annealing step.

#Unit 13. Methods in Biology #PCR #Part B Pointers
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TLS Online TPP Program

#Id: 3016

#Unit 3. Fundamental Processes

ssb mutants have a quick stop phenotype, and are defective in repair and recombination as well as in replication. 
TLS Online TPP Program

#Id: 3017

#Unit 3. Fundamental Processes

All DNA polymerases require a 3'-0H priming end to initiate DNA synthesis.
TLS Online TPP Program

#Id: 3018

#Unit 3. Fundamental Processes

The priming end can be provided by an RNA primer, a nick in DNA, or a priming protein.
TLS Online TPP Program

#Id: 3019

#Unit 3. Fundamental Processes

The priming end can be provided by an RNA primer, a nick in DNA, or a priming protein.
TLS Online TPP Program

#Id: 3020

#Unit 3. Fundamental Processes

For DNA replication, a special RNA polymerase called a primase synthesizes an RNA chain that provides the priming end.
TLS Online TPP Program

#Id: 3021

#Unit 3. Fundamental Processes

Priming of replication on double-stranded DNA always requires a replicase, SSB, and primase.