End-labelling of a gene probe at the 3′ end using terminal transferase. Note that the addition of a labeled dNTP at the 3′ end alters the sequence of the gene probe.

TLS Online TPP Program

#Id: 4279

#Unit 12. Applied Biology

Cell viability is calculated as the number of viable cells divided by the total number of cells.

TLS Online TPP Program

#Id: 4281

#Unit 12. Applied Biology

The best method for cryopreserving cultured cells is storing them in liquid nitrogen in complete medium in the presence of a cryoprotective agent such as dimethylsulfoxide (DMSO) or glycerol

TLS Online TPP Program

#Id: 4535

#Unit 12. Applied Biology

Conventional vaccines consist of whole pathogenic organisms, which may either be killed or live.

TLS Online TPP Program

#Id: 4536

#Unit 12. Applied Biology

In live vaccines, the virulence of pathogens is greatly reduced(attenuation).

TLS Online TPP Program

#Id: 4537

#Unit 12. Applied Biology

Recombinant vaccines

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#Id: 4538

#Unit 12. Applied Biology

Subunit vaccines contain only specific protein from a pathogenic organism. For a virus, this protein is often the coat protein.