End-labelling of a gene probe at the 3′ end using terminal transferase. Note that the addition of a labeled dNTP at the 3′ end alters the sequence of the gene probe.

TLS Online TPP Program

#Id: 777

#Unit 3. Fundamental Processes

FRET allows the detection of position of sigma relative to a site on the DNA to be measured with using separation techniques that might displace sigma from the core enzyme

 

TLS Online TPP Program

#Id: 778

#Unit 3. Fundamental Processes

Termination requires that all hydrogen bonds holding the RNA–DNA hybrid together must be broken, after which the DNA duplex reforms.

TLS Online TPP Program

#Id: 779

#Unit 3. Fundamental Processes

Transcription termination sites are often best characterized in vitro. 

TLS Online TPP Program

#Id: 780

#Unit 3. Fundamental Processes

Two type of Terminators 

1. Intrinsic terminators (Rho-independent terminators )

2. Rho-dependent terminators 

TLS Online TPP Program

#Id: 781

#Unit 3. Fundamental Processes

Intrinsic terminators—

1. Inverted Repeat on DNA 

2. Dyad Symmetry

3. Forms GC–rich hairpin 

4. Base of hairpin 7 U residues (T residues in the DNA) 

TLS Online TPP Program

#Id: 782

#Unit 3. Fundamental Processes

1100 sequences in the E. coli genome that fit these criteria, suggesting more than half of the cell’s transcripts are terminated at intrinsic terminators.