A G-less cassette, or stretch of nucleotides lacking guanines in the nontemplate strand, is inserted just downstream of the promoter. This template is transcribed in vitro with CTP, ATP, and UTP, one of which is labeled, but no GTP.

TLS Online TPP Program

#Id: 512

#Unit 2. Cellular Organization

Repetitive DNA sequences renature faster than non repetitive DNA due to uniformity of sequence.

TLS Online TPP Program

#Id: 513

#Unit 2. Cellular Organization

Cot1/2 is concentration of ssDNA left after time t. T1/2 is time taken for half renaturation.

TLS Online TPP Program

#Id: 514

#Unit 2. Cellular Organization

Low Cot1/2 value means

1. Low complexity with high repeats

2. High complexity with high repeats

3. Low complexity with no repeats

4. no complexity and no repeats

TLS Online TPP Program

#Id: 515

#Unit 2. Cellular Organization

Number of Genes present in per million base pair of genome is called gene density.

TLS Online TPP Program

#Id: 516

#Unit 2. Cellular Organization

Escherichia coli K-12 is 4.6Mb genome with 4400 genes so gene density 950 genes/Mb.

TLS Online TPP Program

#Id: 517

#Unit 2. Cellular Organization

Homo sapiens has 3200 Mb genome in which 20,000 genes are present so Gene density will be 6.25 genes/Mb.