TFIIB, TFIIE, and TFIIH are required to melt DNA to allow polymerase movement. The last factors to join the initiation complex are TFIIE and TFIIH. 

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#Id: 4603

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle

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#Id: 4604

#Unit 13. Methods in Biology

 Propidium iodide-labeled cells in order as
1. Apoptotic cells
2. G1/G0
3. S
4. G2/M 

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#Id: 4605

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

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#Id: 4606

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell

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#Id: 4607

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

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#Id: 4608

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.