The energy for Na+-K+-2Cl- cotransporter is derived from the low intracellular Na+ gradient, which is maintained by the Na+-K+ ATPase

TLS Online TPP Program

#Id: 737

#Unit 3. Fundamental Processes

A two-state assembly model explains melting of 13mer during initiation

TLS Online TPP Program

#Id: 738

#Unit 3. Fundamental Processes

To identify DNA that is in the process of replicating, DNA derived from dividing cells is first cut with a restriction enzyme and separated on a 2D agarose gel.

TLS Online TPP Program

#Id: 739

#Unit 3. Fundamental Processes

DNA strand synthesis requires the four deoxynucleoside triphosphates—dGTP, dCTP, dATP, and dTTP in equal in vivo concentration

TLS Online TPP Program

#Id: 740

#Unit 3. Fundamental Processes

primer:template junction is most important substrate for new DNA synthesis

TLS Online TPP Program

#Id: 741

#Unit 3. Fundamental Processes

The primer is complementary to, but shorter than, the template and have growing 3’ prime terminus

TLS Online TPP Program

#Id: 742

#Unit 3. Fundamental Processes

The phosphodiester bond is formed in an SN2 reaction in which the hydroxyl group at the 3’ end of the primer strand attacks the a-phosphoryl group of the incoming nucleoside triphosphate.